PDE1B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE1B
Enzyme
Human PDE1B
Synonym
Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1B,63 kDa Cam-PDE, PDE1B1, PDES1B)
Substrate
1 µM cGMP, Ca-CaM
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 45,000 |
| Methoxymethyl-IBMX | 56,000 |
| Roliptam | >100,000 |
| Methoxyquinazoline | >100,000 |
Scientific Information
Q01064 (http://www.uniprot.org/uniprot/Q01064)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE1B
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