PDE3A Phoshodiesterase Assay Service

PDE3A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview

CAT#

PDE3A

Enzyme

Human PDE3A

Synonym

cGMP-inhibited 3′,5′-cyclic phosphodiesterase A, CGI-PDE A

Substrate

1 µM cAMP

Assay Properties

Reaction

Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 2,500
Methoxymethyl-IBMX >50,000
Roliptam >50,000
Methoxyquinazoline >50,000

Scientific Information

Q14432 (http://www.uniprot.org/uniprot/Q14432)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE3A

Reference Compound IC50 for PDE3A