PDE3B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE3B
Enzyme
Human PDE3B
Synonym
cGMP-inhibited 3′,5′-cyclic phosphodiesterase B, CGI-PDE B
Substrate
1 µM cAMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 6,400 |
| Methoxymethyl-IBMX | >100,000 |
| Roliptam | >100,000 |
| Methoxyquinazoline | >100,000 |
Scientific Information
Q13370 (http://www.uniprot.org/uniprot/Q13370)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE3B
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