PDE4A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE4A
Enzyme
Human PDE4A
Synonym
cAMP-specific 3′,5′-cyclic phosphodiesterase 4A, DPDE2, PDE46
Substrate
1 µM cAMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 13,900 |
| Methoxymethyl-IBMX | >50,000 |
| Roliptam | >780 |
| Methoxyquinazoline | >1,100 |
Scientific Information
P27815 (http://www.uniprot.org/uniprot/P27815)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE4A
PDF