PDE4A Phoshodiesterase Assay Service

PDE4A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview

CAT#

PDE4A

Enzyme

Human PDE4A

Synonym

cAMP-specific 3′,5′-cyclic phosphodiesterase 4A, DPDE2, PDE46

Substrate

1 µM cAMP

Assay Properties

Reaction

Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 13,900
Methoxymethyl-IBMX >50,000
Roliptam >780
Methoxyquinazoline >1,100

Scientific Information

P27815 (http://www.uniprot.org/uniprot/P27815)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE4A

Reference Compound IC50 for PDE4A