PDE7A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE7A
Enzyme
Human PDE7A
Synonym
High affinity cAMP-specific 3′,5′-cyclic phosphodiesterase 7A, HCP1, TM22
Scientific Information
Q13946 (http://www.uniprot.org/uniprot/Q13946)
Assay Properties
Substrate
0.2 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| BRL-50481 | 5.7 |
| IBMX | >100 |
| Methoxymethyl-IBMX | >100 |
| Rolipram | >100 |
| TC-E 5005 | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services
Reference Compound IC50 for PDE7A
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