PDE8A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE8A
Enzyme
Human PDE8A
Synonym
High affinity cAMP-specific and IBMX-insensitive 3′,5′-cyclic phosphodiesterase 8A
Substrate
1 µM cAMP
Assay Properties
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | >100,000 |
| Methoxymethyl-IBMX | >100,000 |
| Roliptam | >100,000 |
| TC-E 5005 | 58,000 |
Scientific Information
O60658 (http://www.uniprot.org/uniprot/O60658)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE8A
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