PDGFRa Kinase Assay Service

Activity-based PDGFRa kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PDGFRα

Enzyme
Human PDGFRα

HGNC
PDGFRA

Synonym
PDGFR2, CD140a

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.1 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
PP2 18.9
AG1478 306
PDGFR I-III 2.4
D-64406 453
Scientific Information
Q9DE49 (http://www.uniprot.org/uniprot/Q9DE49)

Screening Location
Malvern, USA

Reference compound IC50 for PDGFRα
Reference compound IC50 for PDGFRα