Activity-based PDGFRalpha T674I kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PDGFRalpha T674I
Enzyme
Human PDGFRalpha T674I, mutant
HGNC
PDGFRA (T674I)
Manning ID
PDGFRa
Synonym
PDGFRA, PDGFR2, CD140a, PDGFR-alpha T674I, PDGFRalpha T674I, PDGFRa(T674I)
UniProt
P16234 (PGFRA_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 27 |
| Sorafenib | 400 |
| Sunitinib | 40 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PDGFRalpha T674I |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – PDGFRalpha T674I (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0760-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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