Activity-based PDGFRa(T674I) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDGFRa (T674I)
Enzyme
Human PDGFRa (T674I)
HGNC
PDGFRA (T674I)
Synonym
PDGFR2, CD140a
Scientific Information
Q9DE49 (http://www.uniprot.org/uniprot/Q9DE49)
Assay Properties
Substrates
Peptide substrate, poly[AEKY] (6:2:5:1), 0.2 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.2 |
| PP2 | >100,000 |
| AG1478 | 59 |
| EGFR/ErbB Inhibitor | 17,000 |
| D-64406 | 440 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for PDGFRa (T674I)
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