PDGFRb-TPM3 Kinase Assay Service

Activity-based PDGFRb-TPM3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
-PDGFRb, TPM3

Enzyme
Human TPM3-PDGFRb fusion

HGNC
PDGFRB-TPM3

Synonym
JTK12, PDGFR, CD140B, PDGFR1

Substrates
Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.2
PP2 16
AG1478 340
PDGFR I-III 10
D-64406 69
Scientific Information
TPM3: P06753 (http://www.uniprot.org/uniprot/P06753)PDGFRb: P09619 (http://www.uniprot.org/uniprot/P09619)

Screening Location
Malvern, USA

Reference compound IC50 for PDGFRb-TPM3
Reference compound IC50 for PDGFRb-TPM3

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