Activity-based PDGFRb kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDGFRβ
Enzyme
Human PDGFRβ
HGNC
PDGFRB
Synonym
JTK12, PDGFR, CD140B, PDGFR1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| PP2 | 18.7 |
| AG1478 | 294 |
| PDGFR I-III | 6.3 |
| D-64406 | 93.7 |
Scientific Information
P09619 (http://www.uniprot.org/uniprot/P09619)
Screening Location
Malvern, USA
Reference compound IC50 for PDGFRβ
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