Activity-based PDK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PDK1
Enzyme
Human PDK1, wt
HGNC
PDK1
Manning ID
PDK1
Synonym
PDPK1, PKB-like, PDK1/PDHK1, PDHK1, PDK1
UniProt
Q15118 (PDK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Atypical
Assay Properties
Assay Format
33PanQinaseTM
Substrates
bio-4xCHKT, ATP
Reaction
bio-4xCHKT + 33P-ATP = 33P-bio-4xCHKT + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 11 |
| Sorafenib | 10000 |
| Sunitinb | 4900 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PDK1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – PDK1 |
| Product Number |
0367-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF