Activity-based PDK1/PDHK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDK1/PDHK1
Enzyme
Human PDK1/PDHK1
HGNC
PDK1
Synonym
PKB-like, PDHK1, Pyruvate dehydrogenase kinase isoform 1, Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 mitochondrial
Scientific Information
Q15118 (http://www.uniprot.org/uniprot/Q15118)
Assay Properties
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >100,000 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 9,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
| VER-246608 | 30 |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Kinase Assays.
Reference compound IC50 for PDK1/PDHK1
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