Activity-based PDK4/PDHK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PDK4/PDKH4
Enzyme
Human PDK4/PDKH4
HGNC
PDK4
Synonym
PDHK4, Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial, Pyruvate dehydrogenase kinase isoform 4
Scientific Information
Q16654 (http://www.uniprot.org/uniprot/Q16654)
Assay Properties
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >100,000 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 5,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
| VER-246608 | 35 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for PDK4/PDKH4
PDF