Activity-based PEAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PEAK1
Enzyme
Human PEAK1
HGNC
PEAK1
Synonym
PEAK1, KIAA2002
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.9 |
| PP2 | 19 |
| AG1478 | 883 |
| PDGFR I-III | >100,000 |
| D-64406 | 620 |
Scientific Information
Q9H792 (http://www.uniprot.org/uniprot/Q9H792)
Screening Location
Malvern, USA
Reference compound IC50 for PEAK1
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