Activity-based PHKg2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PHKg2
Enzyme
Human PHKg2
HGNC
PHKG2
Synonym
PHKg2
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.4 |
| Ro 31-8220 | 22,000 |
| GW 5074 | 9,400 |
| H-89 | 8,900 |
Scientific Information
P15735 (http://www.uniprot.org/uniprot/P15735)
Screening Location
Malvern, USA
Reference compound IC50 for PHKg2
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