PI3K (p110a(H1047R)/p85a) Kinase Assay Service

Activity-based PI3K (p110a(H1047R)/p85a) lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview

CAT#
PI3K (p110a(H1047R)/p85a)

Enzyme
Human PI3K (p110a(H1047R)/p85a)

HGNC
PIK3CA (H1047R)-PIK3R1

Synonym
MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha

Substrates
PI(4,5)P2:PS, 10 µM
ATP 10 µM

Assay Properties

Reaction
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
PI-103 3.1
Scientific Information
p110a: P42336 (http://www.uniprot.org/uniprot/P42336)P85a: P27986 (http://www.uniprot.org/uniprot/P27986)

Screening Location
Malvern, USA

Reference compound IC50 for PI3K (p110a(H1047R)/p85a)
Reference compound IC50 for PI3K (p110a(H1047R)/p85a)

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