PI3Kb (p110b/p85a) Kinase Assay Service

Activity-based PI3Kb (p110b/p85a) lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.

Target Overview

CAT#

PI3Kb (p110b/p85a)

Enzyme

Human PI3Kb (p110b/p85a)

HGNC

PIK3CB-PIK3R1

Synonym

PI3K, PI3KBETA, PIK3C1, p110-beta

Substrates

PI(4,5)P2:PS, 10 µM
ATP 10 µM

Assay Properties

Reaction

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ATP = 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
PI-103	6.0

Scientific Information

p110b: P42338 (http://www.uniprot.org/uniprot/P42338)P85a: P27986 (http://www.uniprot.org/uniprot/P27986)

Screening Location

Malvern, USA

Reference compound IC50 for PI3Kb (p110b/p85a)