Activity-based PIK3C3 kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PIK3C3
Enzyme
Human PIK3C3, wt
HGNC
PIK3C3
Manning ID
N/A
Synonym
hVps34, MGC61518, PI3-kinase type 3, PI3K type 3;Vps34, VPS34, PIK3C3, PI3K type 3, Vps34, PI3KC3
UniProt
Q8NEB9 (PK3C3_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PI3K
Assay Properties
Assay Format
ADP-Glo
Substrates
PI, ATP
Reaction
PI + ATP = P-PI + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Wortmannin | 21 |
| LY294002 | 9800 |
| PI-103 | 19000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PIK3C3 |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | Yes |
| IC50 Profiler | IC50 Lipid Kinase Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – PIK3C3 (Activity verified by ADP-Glo – Assay) |
| Product Number | 1160-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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