Activity-based PIK3CA/PIK3R1 kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PIK3CA/PIK3R1
Enzyme
Human PIK3CA/PIK3R1, wt
HGNC
PIK3CA-PIK3R1
Manning ID
N/A
Synonym
MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha, PIK3CA, PI3K-alpha, PIK3CA/PIK3R1, PI3Ka (p110a/p85a)
UniProt
P42336 (PK3CA_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PI3K
Assay Properties
Assay Format
ADP-Glo
Substrates
PIP2:PS, ATP
Reaction
PIP2:PS + ATP = P-PIP2:PS + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Wortmannin | 9 |
| LY294002 | 1700 |
| PI-103 | 26 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PIK3CA/PIK3R1 |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | Yes |
| IC50 Profiler | IC50 Lipid Kinase Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – PIK3CA/PIK3R1 (Activity verified by ADP-Glo – Assay) |
| Product Number | 1161-1165-1 |
| Web Shop | Reaction Biology Online Shop |
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