PIK3CA (Q546K)/PIK3R1 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
PIK3CA (Q546K)/PIK3R1
PIK3CA (Q546K)/PIK3R1
MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently co- expressing NanoLuc-PIK3CA (Q546K) Fusion Vector and PIK3R1 Expression Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-3 and then treated with reference compound PI-103 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
Compounds | IC50 (M) |
---|---|
PI-103 | 3.571E-008 |
Malvern, PA, USA
More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services
Reference compound IC50 for PIK3CA (Q546K)/PIK3R1