Activity-based PIK3CG kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PIK3CG
Enzyme
Human PIK3CG, wt
HGNC
PIK3CG
Manning ID
N/A
Synonym
p120-PI3K, PI3CG, PI3K, PI3Kgamma, PI3K-gamma, p110-gamma, PIK3CG, PI3Kg (p120g), PI3Kg (p110g)
UniProt
P48736 (PK3CG_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PI3K
Assay Properties
Assay Format
ADP-Glo
Substrates
PIP2:PS, ATP
Reaction
PIP2:PS + ATP = P-PIP2:PS + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Wortmannin | 800 |
| LY294002 | 57000 |
| PI-103 | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PIK3CG |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | Yes |
| IC50 Profiler | IC50 Lipid Kinase Profiler |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – PIK3CG |
| Product Number |
1163-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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