Activity-based PIM1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PIM1
Enzyme
Human PIM1
HGNC
PIM1
Synonyms
PIM, Proto-oncogene serine/threonine-protein kinase Pim-1
Scientific Information
P11309 (http://www.expasy.org/uniprot/P11309)
Assay Properties
Substrate
Peptide substrate, [RSRHSSYPAGT], 20 µM ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| Staurosporine | 2.6 |
| Ro 31-8220 | 2.6 |
| GW 5074 | 43 |
| H-89 | 30000 |
| Rottlerin | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition of PIM1
