Activity-based PIM2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PIM2
Enzyme
Human PIM2
HGNC
PIM2
Synonym
PIM2
Substrates
Peptide substrate, [RSRHSSYPAGT], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 29 |
| Ro 31-8220 | 750 |
| GW 5074 | 50 |
| Rottlerin | 6,500 |
Scientific Information
Q9P1W9 (http://www.uniprot.org/uniprot/Q9P1W9)
Screening Location
Malvern, USA
Reference compound IC50 for PIM2
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