Activity-based PIP5K1A kinase assay for compound screening and profiling via ADP-Glo – assay technology
Target Overview
RBE Target ID
PIP5K1A
Enzyme
Human PIP5K1A, wt
HGNC
PIP5K1A
Manning ID
N/A
Synonym
PIP5K1-alpha, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha
UniProt
Q99755 (PI51A_HUMAN)
Kinase Type
Lipid kinase
Kinase Group
PIP5K
Assay Properties
Assay Format
ADP-Glo
Substrates
PI:PS, ATP
Reaction
PI:PS + ATP = P-PI:PS + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Wortmannin | 100000 |
| LY294002 | 100000 |
| PI-103 | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PIP5K1A |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | Yes |
| IC50 Profiler | IC50 Lipid Kinase Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – PIP5K1A (Activity verified by ADP-Glo – Assay) |
| Product Number | 1231-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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