Activity-based PIP5K1A lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
PIP5K1A
Enzyme
Human PIP5K1A
HGNC
PIP5K1A
Synonym
PIP5K1-alpha, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha
Substrates
PI(4)P:PS, 50 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
1-phosphatidyl-1D-myo-inositol 4-phosphate + ATP = 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PIK-93 | 88,000 |
Scientific Information
Q99755 (http://www.uniprot.org/uniprot/Q99755)
Screening Location
Malvern, USA
Reference compound IC50 for PIP5K1A
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