PKA-DNAJB1 Kinase Assay Service

Activity-based PKA-DNAJB1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#

PKA-DNAJB1

Enzyme

Human DNAJB1-PKA Fusion Protein

HGNC

PRKACA-DNAJB1

Synonym

PKACa, Hsp40, Sis1, RSPH16B
DNAJB1: DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, hDj-1, DNAJ1, HDJ1, HSPF1 
PKA: cAMP-dependent protein kinase catalytic subunit alpha, PRKACA, PKACA, KAPCA

Substrates

Peptide substrate, [LCGRTGRRNSI], 100 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.3
Ro 31-8220 230
GW5074 40,000
H-89 21
Rottlerin >100,000

Scientific Information

DNAJB1: P25685 (http://www.uniprot.org/uniprot/P25685)
PKA: P17612 (http://www.uniprot.org/uniprot/P17612)

Screening Location

Malvern, USA

Reference compound IC50 for PKA-DNAJB1

Reference compound IC50 for PKA-DNAJB1

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