Activity-based PKAcb kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKAcb
Enzyme
Human PKAcb
HGNC
PRKACB
Synonym
PKAcb, MGC41879, MGC9320, PKA c-beta
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.1 |
| Ro 31-8220 | 250 |
| H-89 | 220 |
Scientific Information
P22694 (http://www.uniprot.org/uniprot/P22694)
Screening Location
Malvern, USA
Reference compound IC50 for PKAcb
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