Activity-based PKCb2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCb2
Enzyme
Human PKCb2
HGNC
PRKCB
Synonym
PRKCB1, PKCB, PRKCB2, MGC41878, PKC-beta, PKC-beta2
Substrates
Peptide substrate [ERMRPRKRQGSVRRRV], Lipid activator, 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.4 |
| Ro 31-8220 | <0.1 |
| GW5074 | 3,000 |
| H-89 | 930 |
| Rottlerin | >100,000 |
Scientific Information
P05771-2 (http://www.uniprot.org/uniprot/P05771-2)
Screening Location
Malvern, USA
PDF