Activity-based PKCepsilon kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCepsilon
Enzyme
Human PKCepsilon
HGNC
PRKCE
Synonym
PKCE, nPKC-epsilon, PKC-epsilon
Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM, Lipid activator,
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.18 |
| Ro 31-8220 | 0.38 |
| GW5074 | 8,400 |
| H-89 | 93 |
| Rottlerin | >100,000 |
Scientific Information
Q02156 (http://www.uniprot.org/uniprot/Q02156)
Screening Location
Malvern, USA
Reference compound IC50 for PKCepsilon
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