Activity-based PKCg kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCg
Enzyme
Human PKCg
HGNC
PRKCG
Synonym
PKCC, PKCG, SCA14, MGC57564, PKC-gamma
Substrates
Peptide substrate [ERMRPRKRQGSVRRRV], 20 µM, and Lipid activatorATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.5 |
| Ro 31-8220 | <1.0 |
| GW5074 | 2,200 |
| H-89 | 280 |
| Rottlerin | >100,000 |
Scientific Information
P05129 (http://www.uniprot.org/uniprot/P05129)
Screening Location
Malvern, USA
Reference compound IC50 for PKCg
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