Activity-based PKCgamma kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PKCgamma
Enzyme
Human PKCgamma, wt
HGNC
PRKCG
Manning ID
PKCg
Synonym
PKC-gamma, PKCC, PKCG, SCA14, MGC57564, PRKCG, PKCgamma, PKCg
UniProt
P05129 (KPCG_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
3xChocktide-PKC(19-31), ATP
Reaction
3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 5 |
| Sorafenib | 10000 |
| Sunitinib | 11000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PKCgamma |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – PKCgamma (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0204-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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