Activity-based PKCmu/PRKD1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCmu
Enzyme
Human PKCmu
HGNC
PRKD1
Synonym
PKD1, PKD, PKCM, PKc-MU
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.5 |
| Ro 31-8220 | 210 |
| GW 5074 | 5,700 |
| H-89 | 910 |
Scientific Information
Q15139 (http://www.uniprot.org/uniprot/Q15139)
Screening Location
Malvern, USA
Reference compound IC50 for PKCmu/PRKD1
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