Activity-based PKCnu/PRKD3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCnu/PRKD3
Enzyme
Human PKCnu/PRKD3
HGNC
PRKD3
Synonym
PKD3, EPK2, PKc-NU, PKD3, PRKCN
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.6 |
| Ro 31-8220 | 84 |
| GW 5074 | 4,000 |
| H-89 | 1,300 |
Scientific Information
O94806 (http://www.uniprot.org/uniprot/O94806)
Screening Location
Malvern, USA
Reference compound IC50 for PKCnu/PRKD3
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