Activity-based PKCzeta kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKCzeta
Enzyme
Human PKCzeta
HGNC
PRKCZ
Synonym
PKC2
Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 87 |
| Ro 31-8220 | 138 |
| H-89 | 3,500 |
Scientific Information
Q05513 (http://www.uniprot.org/uniprot/Q05513)
Screening Location
Malvern, USA
Reference compound IC50 for PKCzeta
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