Activity-based PKD2/PRKD2 (G848E) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKD2/PRKD2 (G848E)
Enzyme
Human PKD2/PRKD2 (G848E)
HGNC
PKD2/PRKD2 (G848E)
Synonym
Serine/threonine-protein kinase D2, nPKC-D2, KPCD2, PRKD2
Scientific Information
Q9BZL6 (http://www.uniprot.org/uniprot/Q9BZL6)
Assay Properties
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.8 |
| Ro 31-8220 | 210 |
| GW5074 | 2,900 |
| H-89 | 1,100 |
| Rottlerin | 20,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for PKD2/PRKD2 (G848E)
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