Activity-based PKD2/PRKD2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKD2
Enzyme
Human PKD2
HGNC
PRKD2
Synonym
PKD2, HSPC187
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.47 |
| Ro 31-8220 | 263 |
| GW 5074 | 4,000 |
| H-89 | 2,300 |
Scientific Information
Q9BZL6 (http://www.uniprot.org/uniprot/Q9BZL6)
Screening Location
Malvern, USA
Reference compound IC50 for PKD2/PRKD2
PDF