Activity-based PKG1a kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKG1α
Enzyme
Human PKG1α
HGNC
PRKG1
Synonym
PKG1, PGK, PRKG1B, PRKGR1B
Substrates
Peptide substrate, [LRRASLG], 100 µM
ATP 1 µM
ATP 1 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.86 |
| Ro 31-8220 | 29 |
| GW 5074 | 10,000 |
| H-89 | 55 |
Scientific Information
Q13976 (http://www.uniprot.org/uniprot/Q13976)
Screening Location
Malvern, USA
Reference compound IC50 for PKG1α
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