Activity-based PKMzeta kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PKMzeta
Enzyme
Human PKMzeta, wt
HGNC
PRKCZ (aa184-592)
Manning ID
PKCz
Synonym
PRKCZ, PKM-zeta, nPKC-zeta, PKC2, PKC-zeta wt aa184-592 (PKM-zeta), PKMzeta, PKC-zeta
UniProt
Q05513 (KPCZ_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
3xChocktide-PKC(19-31), ATP
Reaction
3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 123 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PKMzeta |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – PKMzeta |
| Product Number |
1240-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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