PKMzeta Kinase Assay Service

Activity-based PKMzeta kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
PKMzeta (N-term (aa1-183) truncated PKCzeta)

Enzyme
Human PKMzeta

HGNC
PRKCZ

Synonym
nPKC-zeta, PKC2

Substrates
Peptide substrate, [ERMRPRKRQGSVRRRV], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 67
Ro 31-8220 98
GW 5074 24,000
H-89 9,500
Rottlerin >100,000
Scientific Information
Q05513 (http://www.uniprot.org/uniprot/Q05513)

Screening Location
Malvern, USA

Reference compound IC50 for PKMzeta
Reference compound IC50 for PKMzeta