Activity-based PKN1/PRK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKN1
Enzyme
Human PKN1
HGNC
PKN1
Synonym
DBK, PKN, PAK1, PRK1, PRKCL1, MGC46204
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| Ro 31-8220 | 2.3 |
| GW 5074 | 5,600 |
| H-89 | 79 |
Scientific Information
Q16512 (http://www.uniprot.org/uniprot/Q16512)
Screening Location
Malvern, USA
Reference compound IC50 for PKN1/PRK1
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