Activity-based PKN2/PRK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKN2
Enzyme
Human PKN2
HGNC
PKN2
Synonym
PRK2, PRKCL2, PAK-2
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.2 |
| Ro 31-8220 | 210 |
| H-89 | 1,200 |
Scientific Information
Q16513 (http://www.uniprot.org/uniprot/Q16513)
Screening Location
Malvern, USA
Reference compound IC50 for PKN2/PRK2
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