Activity-based PKN3/PRK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PKN3
Enzyme
Human PKN3
HGNC
PKN3
Synonym
PRK3, PKNbeta, RP11-545E17.1
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.7 |
| Ro 31-8220 | 340 |
| GW5074 | >50,000 |
| H-89 | 96 |
| Rottlerin | >50,000 |
Scientific Information
Q6P5Z2 (http://www.uniprot.org/uniprot/Q6P5Z2)
Screening Location
Malvern, USA
Reference compound IC50 for PKN3
PDF