Activity-based PLK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
PLK2
Enzyme
Human PLK2
HGNC
PLK2
Synonym
SNK
Substrates
Casein, 1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 740 |
| Ro 31-8220 | 49,000 |
| GW 5074 | 19,000 |
Scientific Information
Q9NYY3 (http://www.uniprot.org/uniprot/Q9NYY3)
Screening Location
Malvern, USA
Reference compound IC50 for PLK2
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