PP2C-alpha (PPM1A) Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PP2C-alpha
Enzyme
Human PP2C-alpha
Synonyms
PPPM1A, PPM1A, Protein phosphatase 2C isoform alpha (PP2C-alpha), Protein phosphatase 1A
Scientific Information
P35813 (https://www.uniprot.org/uniprotkb/P35813)
Assay Properties
Substrate
Fluorogenic substrate DiFMUP, 50 μM
Reaction
[protein]-serine/threonine phosphate + H₂O = [protein]-serine/threonine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compound | IC50 (mM) |
|---|---|
| PTP1B Inhibitor | 4.6 |
| NSC 95397 | 16.4 |
| Sanguinarine chloride | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Phosphatase Assay Services for Drug Discovery
Reference compound inhibition of PP2C-alpha (PPM1A)
