Activity-based PRDM9 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRDM9
Enzyme
Human PRDM9
Synonym
Histone-lysine N-methyltransferase PRDM9, PR domain zinc finger protein 9, PR domain-containing protein 9, PFM6
Substrates
Histone H3, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 160,000 |
| Sinefugin | >200,000 |
| BIX 01294 | >200,000 |
| Chaetocin | 3,400 |
Scientific Information
Q9NQV7 (http://www.uniprot.org/uniprot/Q9NQV7)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for PRDM9
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