Activity-based PRKG1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PRKG1
Enzyme
Human PRKG1, wt
HGNC
PRKG1 (a)
Manning ID
PKG1
Synonym
CGKI, MGC71944, PGK, PRKG1B, PRKGR1B, cGKI-BETA, cGKI-alpha, PKG1, PRKG1, MGC71944, cGKIBETA, cGKI-alph, PKG1a
UniProt
Q13976 (KGP1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
3xChocktide-PKC(19-31), ATP
Reaction
3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| Sorafenib | 10000 |
| Sunitinib | 16000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PRKG1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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