Surface Plasmon Resonance (SPR) technique for evaluating direct binding of PRMT1 to molecular inhibitors
PRMT1
PRMT1
Protein arginine N-methyltransferase 1
PRMT5: Q14744
(https://www.uniprot.org/uniprotkb/Q99873/entry)
Produced at Reaction Biology. Catalog # HMT-11-119
(http://reactionbiology-com.3dcartstores.com/PRMT1-Methyltransferase_p_15.html)
Malvern, PA, USA
More information can be found on our website SPR Assay Services for Drug Discovery
Binding of reference compound (SAH) to PRMT1
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a steady state affinity model determine binding affinity (KD).
Binding of GSK3368715 to PRMT1 in the presence of SAM
The data was collected on a Biacore 8K using single-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model to determine binding affinity (KD).