Activity-based PRMT4 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT4
Enzyme
Human PRMT4
Synonym
Protein arginine N-methyltransferase 4, Histone-arginine methyltransferase CARM1, Coactivatorassociated arginine methyltransferase 1, CARM1
Substrates
Histone H3, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 120 |
| Sinefugin | 100 |
Scientific Information
Q86X55 (http://www.uniprot.org/uniprot/Q86X55)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for PRMT4
PDF