Activity-based PRMT6 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT6
Enzyme
Human PRMT6
Synonym
Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6, ANM6
Substrates
GST-GAR, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 120 |
| Sinefugin | 260 |
| BIX01294 | >200,000 |
| Chaetocin | >200,000 |
Scientific Information
Q96LA8 (http://www.uniprot.org/uniprot/Q96LA8)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for PRMT6
PDF